Overview of Bacterial and Yeast Systems for Protein Expression

Over the past decade variety of hosts and vector systems for recombinant protein expression has increased dramatically. Researchers now select from among mammalian, insect, yeast, prokaryotic hosts, number vectors available use in these organisms continues to grow. With availability cDNAs coding sequencing information, it is certain that other, yet be developed will important future. Despite development eukaryotic systems, E. coli remains most widely used host expression. Optimization been carried out by varying simple parameters such as vectors, strains, media composition, growth temperature. Recombinant gene often only viable route large-scale production authentic, post translationally modified proteins. It becoming increasingly easy find a suitable system overexpress virtually any product, provided properly engineered into an appropriate vector.